Pdf alloxaninduced diabetes, a common model for evaluating the. Blood glucose lowering activity of aloe based composition. The present study evaluated the efficacy of an aqueous flaxseed extract afe to correct alloxan induced diabetes in mice. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Antidiabetic activity of methanolic bark extract of. Treatment was done in alloxan induced model for 24. Research article alloxan induced diabetes mellitus in wistar. The hitherto unknown glucose regulating role of three vegetable peels from cucurbitaceae family was evaluated. Procurement and identification of plant material the bark of plant was collected from the herbal nature.
Alloxan is a pancreatic beta cellselective toxin that induces diabetes in rodents by generating cytotoxic free radicals. In each pancreas of normal mice, there were a few very large islets with diameters between 400 and 600. Antidiabetic effects of rice hull smoke extract in alloxan. Dose optimization of alloxan for diabetes in albino mice. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. For the management of the carbohydrate metabolism in diabetes by the cos, the amount of glucose in blood along with. This paper presents results of experimental study of cerebral blood flow microcirculation in mice with alloxan induced diabetes using temporal laser speckle imaging tlsi. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. The mechanism of dna damage induced by alloxan was investigated using 32plabeled human dna fragments. Antidiabetic activity of ethanolic extract of tubers of.
This study evaluated the longterm effects of alloxaninduced diabetes in rat liver. Effects on hepatic enzymes in alloxan induced diabetic rats. Whether after 10 to 60 min incubation of pieces of pancreas in the presence of 214c alloxan or 60 min to 24 h after intravenous injection of 214c alloxan to control and insulintreated stz rats, the radioactive. Mice with glucose levels above 150 mgdl were considered diabetic and used for the efficacy study xu et al. Characteristics of the epidermis and stratum corneum of. Various developments from gene transplants to invitro experiments on mice have emerged 7. Consistently, ipgtt performed on day 9 showed that alloxaninduced glucose intolerance was abrogated in nox2. Nox2 deficiency protects against streptozotocininduced. B activation and protects against alloxaninduced diabetes in cd1 mice. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. May 24, 2014 insulin dependent diabetes was induced by administering a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg to cd1 mice. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species ros in a cyclic redox reaction with its reduction product, dialuric acid.
Pdf experimental diabetes induced by alloxan and streptozotocin. Research design and methods this study evaluated how stz induced diabetes. It has been widely used to induce experimental diabetes in animals such as rabbits, rats, mice and dogs with different. Antihyperglycemic effects of fermented and nonfermented. Diabetes mellitus was induced by alloxan monohydrate 120 mgkg, i. Hypoglycemic activity of aqueous extract of pleurotus pulmonarius. Alloxaninduced diabetes triggers the development of.
Hyperglycaemic rats were treated with or without oral. Effects of streptozotocin and alloxaninduced diabetes mellitus on mouse follicular and early embryo development. Antidiabetic study of fjmk in alloxan induced diabetic model induction of experimental diabetes diabetes was induced in mice by a single injection of alloxan monohydrate 150 mg kg i. Sensitization to alloxaninduced diabetes and pancreatic. Effects of diabetes mellitus induced by alloxan on the. Antihyperglycemic and antihyperlipidemic effects of.
Hyperglycaemia was induced in fasted wistar rats with alloxan 150 mgkg body weight. The control of hyperglycemia by estriol and progesterone in. Immunosuppressive effects of streptozotocininduced. Objective streptozotocin stz is the most widely used diabetogenic agent in animal models of islet transplantation. Thus, the aim of the present study was to investigate the antidiabetic activity of methanolic bark extract of albizia odoratissima in alloxan induced diabetic mice. The mechanisms of alloxan and streptozotocin induced diabetes. African journal of pharmacy and pharmacology evaluation. The induction of alloxan induced diabetes was confirmed by measuring blood glucose levels of each mouse after 7 days by following the blood glucose monitoring after alloxan administration. Betacellulin btc, a member of the epidermal growth factor family, is expressed predominantly in the human pancreas and induces the differentiation of a pancreatic acinar cell line ar42j into insulinsecreting cells, suggesting that btc has a physiologically important role in the endocrine pancreas.
Furthermore, alloxan inhibits glucokinase, a shcontaining protein essential for insulin secretion induced by glucose. Genetic and surgical models were rarely used because they required highly technical skills and the percentage of animals lost during the procedures were higher. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Alloxan administered doses range from 50 to 200 mgkg in mice and 40 to 200 mgkg in rats, and they are dependent on the chosen strain and the route of. We were able to consistently microdissect islets from both normoglycemic and alloxan diabetic mice. The mechanisms of alloxan and streptozotocininduced. Fresh liver weight and its relationship with body weight were obtained, and liver. Rats were made to fast prior to alloxan administration. However, the immunomodifying effects of stz and the ensuing hyperglycemia on lymphocyte subsets, particularly on t regulatory cells tregs, remain poorly understood. Alloxaninduced diabetic mice, antidiabetic activity, oral glucose tolerance. They stated that the this dose of alloxan agents required for inducing dm can be toxic if it administrate by the route of intravenous iv 5,12. Even so, dermatologists still lack an animal model of diabetes that enables the direct evaluation of the various functional properties of the skin. It is better to standardize it first by jain et al. Alloxaninduced diabetes, a common model for evaluating.
Comparison of cerebral microcirculation of alloxan diabetes. Alloxan induced diabetes in you can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more. Experimental diabetes induced by alloxan and streptozotocin. In a preliminary study, effects of ethanolic extracts of cucurbita pepo, cucumis sativus and praecitrullus fistulosus peels were studied at 250 and 500 mg kg. Autoxidation of dialuric acid generates superoxide radicals. Vascular reactivity in arterioles from normal and alloxan. Effects of streptozotocin and alloxaninduced diabetes mellitus on. In diabetic rats, the fasting blood glucose level, indexes of heart and liver, and levels of cholesterol and triglyceride in serum and mda in heart.
Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. Champ lentinaceae on serum glucose in alloxaninduced diabetic mice. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Antidiabetic activity of ipomoea batatas leaves extract. Glucose prevents the development of diabetes induced by alloxan. Diabetes was induced using intraperitoneal injection of alloxan. Diabetes mellitus induces many pathophysiologic changes in the skin. Group 1 was the normal control mice that received distilled water as placebo while groups 26 were diabetic mice. Alloxaninduced diabetes model diabetes was induced by a single intraperitoneal injection of 150 mgkg monohydrated alloxan sigma, st. Antidiabetic potency, alloxaninduced diabetic rats, aloe vera.
Antidiabetic effects of rhse were evaluated in both the rat insulinoma1 cell line ins1 and diabetic icr mice induced by intraperitoneal ip injection of alloxan. The responses are accompanied by corresponding inverse changes in. The incidence of diabetes at 7 days after intraperitoneal injection with 120 mgkg. The hemimandibles were removed and submitted to radiographical and histopathological procedures. Here, we investigated the influence of diabetes on allergic airway inflammation in a model of experimental asthma in mice. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57. Diabetes was induced by intravenous injection of alloxan into 12 hfasted aj mice, followed by subcutaneous sensitization with ovalbumin ova and aluminum hydroxide aloh 3, on days 5 and 19 after diabetes induction. Diabetic control mice, diabetes was induced by alloxan 150 mgkg i. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals.
Diabetes was induced in wistar rats n 25 by intravenous administration of alloxan 42 mgkg and were analyzed at 1, 3, 6, 9 and 12 months after diabetes induction. Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal effects on other structures. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l. The effects of dosage and the routes of administrations of. After 48 h, the animals showing serum glucose levels. Alloxan definition of alloxan by medical dictionary. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. Also, this convenient method of producing a permanent experimental pancreatic diabetes has stimulated research in the fields of carbohydrate metabolism and the role of insulin in metabolism. Antidiabetic potential of caralluma europaea against alloxan induced diabetes in mice article pdf available in saudi journal of biological sciences 266 june 2018 with 369 reads. Amaranthus dubious on alloxan induced diabetic mice.
Hyperglycemia was not observed in the control mice in both groups n 15 to 20 animals in each group. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. Alloxaninduced diabetes delays repair in a rat model of closed tibial. After 12 h, a 10% glucose solution was offered to the animals to prevent hypoglycemia 27,28. A correlative study with antihyperlipidemic and antioxidative activity. Dec 18, 2007 alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter.
Feb 28, 2020 the present study evaluated the efficacy of an aqueous flaxseed extract afe to correct alloxan induced diabetes in mice. Materials and methods plant material and preparation of extract the leaves of amaranthus dubious were collected in july 2012 from area around gondar college of medicine health sciences, university of gondar, ethiopia. Groups included diabetic mice treated with crude aqueous extract of artemisia afra at dose of 500 mgkg, 750 mgkg, and mgkg po. Efficacy of chitooligosaccharides for the management of diabetes in alloxan induced mice. Alloxaninduced diabetes and bone repair 373 issn 0100. This study evaluated the longterm effects of alloxan induced diabetes in rat liver. Treatment of alloxaninduced diabetic rats with metformin or. Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet. Antidiabetic activity of methanolic bark extract of albizia. Thirty nondiabetic control rats nc and 30 untreated. Alloxan has been widely used to induce experimental dm in animals such as rabbits, mice and dogs with different grades of disease severity by varying the dose of alloxan used 8 causing alloxan.
Sugarlowering drugs for type 2 diabetes mellitus and. Streptozotocin and alloxan diabetes in mice blood glucose, mg 100 ml 300 alloxan 100 streptozotocin streptozotoc 1. The anhydrous derivative ocnhco 2 co is also known as well as a dimeric derivative. The results showed that thp could decrease the blood glucose, tc, tg, ldl. However, others workers reported its different doses for induction of diabetes. Alloxan is an oxidative stress agent that is relatively specific to, and destroys the insulinproducing pancreatic.
In the present study, the protective mechanism of glucose against alloxaninduced. Cuiidependent dna damage increased with the concentration of alloxan and nadh. Improvement of diabetes symptoms and complications by an. Effect of aloe vera leaf extract on alloxan induced diabetes. Hyperglycemia enhances the production of reactive oxygen species, leading. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation. The mechanisms of alloxan and streptozotocininduced diabetes. In this study, we examined the in vivo effect of recombinant human btc rhbtc on glucose. Our group induced two types of an experimental type 1 diabetes model in hairless mice by administering either streptozotocin or alloxan, in order to examine the properties of the. Comparison of streptozotocin and alloxaninduced diabetes. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. The alr alloxan induced diabetes resistant inbred strain was created in japan by selective inbreeding of crj. Research article alloxan induced diabetes mellitus in.
Alloxaninduced diabetes causes morphological and ultrastructural. Protective role of three vegetable peels in alloxan. Diabetes was induced using intraperitoneal injection of alloxan 100 mgkg, 0. Mice n 15group were fasted overnight before measurements were taken for fasting blood glucose levels at preinduction, week0 baseline, week2 and week4 after treatment. The effects of alloxan on the histology of the pancreas. Fasting blood glucose fbg levels were measured 5 days after alloxan injection, and on the 3rd, 7th, 10th, and the 14th day after treatment administration. Alloxan induced diabetes and cytokine mobilization of bone marrow chimeric mice. Hyperglycemia enhances the production of reactive oxygen species. The mechanism of alloxan and streptozotocin action in b cells. Alloxan induced experimental diabetic swiss albino mice fgb.
Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. Prior to administration of alloxan, mice were fasted overnight but given water ad libitum. Pdf efficacy of chitooligosaccharides for the management. Alloxan is known to induce diabetes in experimental animals through destruction of insulinproducing 3cells of pancreas. Jain and arya in 2011 state that doses of drugs, routes of administration, are more important issue in duration and severity of diabetes and methodology in alloxan induced diabetic models. For obvious technical reasons, it was much easier to locate and prepare large islets fig. C, insulin levels, and enhance the activities of antioxidant enzyme. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. Alloxan induced diabetes is one of the widely used model to induce type i diabetes. This research will contribute to the understanding of the etiology of iddm and may lead to the development of better strategies for disease prevention. The present study evaluates the effects of chitooligosaccharides cos for the management of alloxan induced diabetes in mice.
These are some of the earliest known organic compounds. Alloxaninduced diabetes is a form of insulin dependent diabetes mellitus that occurs as. We report herein the findings of this research work. Hypoglycemic effect of aqueous and methanolic extract of. In research it is used for induction of type 1 diabetes. The uptake of 214c alloxan by the pancreatic gland was investigated in control and streptozotocin induced diabetic stz rats, using both in vitro and in vivo techniques. The mice were randomly assigned into six different groups n 8. Protective mechanism of glucose against alloxaninduced. After injection of alloxan at the dose of 40 mgkg, only a slight transient hyperglycemia.
Fresh liver weight and its relationship with body weight were obtained, and liver tissue was. Streptozotocin and alloxandiabetes in mice sciencedirect. Alloxan is a urea derivative which causes selective necrosis of the. Antihyperglycemic and antihyperlipidemic effects of aqueous. Zn supplementation potentially preventing alloxan induced diabetes was reported initially by tadros et al. They also exhibit a variety of biological activities. The resulting insulinopenia causes a state of experimental diabetes mellitus called alloxan diabetes 4, 8, 9. Sir, we read with interest the article titled anomalies in alloxan induced diabetic model. Induction of diabetes diabetes mellitus was induced by intraperitoneal injection of alloxan monohydrate 120 mgkg body weight7. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as. These were administered orally 500 mg extractkg body weight to alloxan induced diabetic female balbc mice to determine their hypoglycemic effects. Sensitization to alloxaninduced diabetes and pancreatic cell.
Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Consistently, ipgtt performed on day 9 showed that alloxan induced glucose intolerance was abrogated in nox2. The mice were randomly assigned into six different groups 8. The induction of diabetes mellitus aims to use animals under. In this study, we examined the in vivo effect of recombinant human btc rhbtc. Nov 30, 2011 this study investigated the protective effect of a liquid rice hull smoke extract rhse against diabetes in alloxan induced diabetic mice. Alloxan, sometimes referred to as alloxan hydrate, refers to the organic compound with the formula ocnhco 2 coh 2. The mechanism of alloxan and streptozotocin action in b. Hypoglycemic effects in alloxaninduced diabetic rats of the.
Additionally, a direct effect of glucose water solution concentration 20% and 45% on blood flow microcirculation was studied. Animals models for studying diabetes mellitus citeseerx. This study was undertaken to investigate the preventive mechanism of amomum xanthoides extract against the development of alloxan induced diabetics of mice. The incidence of diabetes at 7 days after intraperitoneal injection with 120 mgkg bw of alloxan alx 120 was 0% in the wildtype mice group, 7. Alloxaninduced diabetes, a common model for evaluating the. Of immediate interest was the question of whether permanent diabetes could be produced in other animals. Pdf antidiabetic potential of caralluma europaea against. The longterm effects of subdiabetogenic doses of alloxan monohydrate alloxan induced a marked albeit transient hyperglycemia. Alloxaninduced diabetes mellitus how is alloxaninduced. Effects on hepatic enzymes in alloxan induced diabetic rats diabetes mellitus is the most common endocrine disorder of man, whose devastating effect is increasing by the day and severity almost at epidemic level.
It has been successfully induced in a variety of animal species. Please contact our customer care department to inquire about current availability of this chemical, and an estimated shipping and delivery time to the phillippines. The effect of intravenous injection of alloxan monohydrate 70 mgkg and streptozotocin 200 mgkg on blood glucose levels in nonfasted female nm ri mice. The current state of the art article pdf available in journal of pharmacological and toxicological methods 78.
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